Our High Sensitivity Immunoassay Proteomics Platform
Designed by end-users for end-users in disease detection, startification and translational research, Aeirtec have integrated their proprietary assay biochemistry, patented standard curve analysis equations and immobilized bead technology to produce their novel proteomics platform. The technology provides: (1) high marker measurement sensitivity (low femtogram/ml) without the need for added NGS, (2) extended dynamic range by at least an order of magnitude (3) maintenance of assay linearity in the lowest concentratons. Practically, the system combines ELISA plate handling simplicity, fluidics-free plate analysis in under 10 minutes, 150 analyte multiplexing, simple customization and permanent repeat plate analysis for QC purposes. Aeirtec currently use this technology in their collaborations and will be soon launching products of this technology for end-user use.
A unique method of calibration curve generation and curve fitting, derived from advances in understanding antibody-analyte interaction, that enables calibration curve analysis and linearity at very low analyte concentrations, providing optimized interpolation for sample mediator quantification.
Why assay sensitivity is so important in demonstration of efficacy and mechanism
Many initiating mediators of cascade responses , such as immune/inflammatory response initiating markers (eg. TNF, IFN, IL-1, IL-2, IL-4, IL-6) are primary candidates for inclusion in study assay panels, because of their hierarchy dominant actions.
However, these initiating markers typically exist at low abundance levels and below the level of quantification of many standard immunoassays.
Aeirtec’s proteomics platform avoids the loss of subject data, typical in standard assay sensitivity, by applying their integration of patented and proprietary biochemical assay techniques.
