Why is assay sensitivity so important in demonstration of efficacy and mechanism?
Many initiating mediators of cascade responses , such as immune/inflammatory response initiating markers (eg. TNF, IFN, IL-1, IL-2, IL-4, IL-6) are primary candidates for inclusion in study assay panels, because of their hierarchy dominant actions.
However, these initiating markers typically exist at low abundance levels and below the level of quantification of many standard immunoassays.
Aeirtec avoids the loss of subject data, typical of standard assay sensitivity, by applying their integration of patented and proprietary biochemical assay techniques.
By increasing assay sensitivity Aeirtec increases the capacity for capturing low abundance critical markers by an order of magnitude.
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An Aeirtec-designed buffer that reduces assay ‘noise’ from (a) nonspecific binding and (b) heterophilic antibody activity.
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A second Aeirtec-designed buffer that generates calibration curves that match the sample-containing fluid behaviour (matrix-matched)
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unique method of calibration curve generation and curve fitting, derived from advances in understanding antibody-analyte interaction, that enables calibration curve analysis at very low analyte concentrations, providing optimised interpolation for sample mediator quantification.
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Why is assay sensitivity so important in demonstration of efficacy and mechanism of action?