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Ultra High Sensitivity in Multiplex ELISA
Aeirtec provides a modular integrated service of (1) ultrasensitive multiplex ELISA marker measurement, (2) custom marker panel design and (3) data interpretation through biostatistics. This service is conducted according to the principles of GLP and ISO 17025:2005 compliance in conjunction with internal and external independent Quality Assurance
Custom Marker Panels In ELISA Multiplex Service
As an option in the Integrated Multiplex ELISA Service we provide help with the design, or entirely design multiplex panels for a wide range of clincial and basic research studies.
Using commercially available and validated antibodies we also generate assays for novel proteins or peptides for incorporation into multiplex panel construction and validation. Therefore, markers from a range of different physiological systems can be brought together within the same multiplex panel.
Data Interpretation Through Biostatistics
Aeirtec has focused assay development on generation of the highest sensitivity customised multiplex ELISA service activity available. This avoids the common problem of ‘below level of detection’ data and thereby maintains study power through retention of sample data. Aeirtec has developed and incorporated proporietary unique ELISA multiplex assay technology in our Multiplex ELISA service to achieve this goal.
With the incorporation of such high sensitivity, multiplexing is still the most cost efficient immunoassay route for even low numbers of markers within samples and remains a cheaper option than single ELISA kit assay.
We apply biostatistics to multiplex ELISA data. As an example, multivariate analysis-guided hierarchical classification analysis is conducted to monitor marker group activity as integrated constellations.
This statisitical integration to produce marker patterns reveals the greater power of integrated marker groups in measurement of efficacy, disease stratification, diagnostics and prediction and characterisation of biological states and events.